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Schematic representation of the bicistronic constructs used for transfection of QT6 cells. Po CMV, cytomegalovirus early promoter; SV40, simian virus 40. (A and B) DNA constructs direct synthesis of bicistronic capped RNAs. However, the presence of a stable stem-loop structure just upstream of neo in the construct shown in panel B is thought to inhibits neo expression (36). (C) Sequence and computer-predicted structure of the stem-loop structure (ΔG = −50 kcal/mol).
