FIG. 7.

Synthesis of β-galactosidase directed by bicistronic neo-LacZ RNAs in QT6 cells. Transfection of the bicistronic plasmids was performed by the Fugene method (Materials and Methods). Two days later, β-galactosidase-specific activity was measured on cellular extracts and neo-lacZ RNA was quantified by slot blot analysis. Efficiency of lacZ translation for each bicistronic RNA is given as the ratio of β-galactosidase-specific activity per arbitrary unit of bicistronic neo-lacZ mRNA. Results are the averages of the results from two independent experiments.