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Detection of NORVA membrane proteins from various cells. The membrane fraction was prepared by homogenization (Caco-2, HeLa, HepG2, N-MuLi, and CV-1) or treatment with detergent (1% NP-40 [293T, CHO, Tn5, and Sf9]). Approximately 20 μg of each cell membrane lysate was used for VOPBA. Lanes 1 to 9 contained Caco-2, 293T, N-MuLi, CHO, Tn5, CV-1, HeLa, HepG2, and Sf9, respectively. The membrane was incubated with 200 μg of unlabeled rUEVs. The NORVA protein was indicated by an arrowhead.
