FIGURE 4:

Quantification of S-layer removal and outer-membrane ruffling. (A) Cartoon demonstration of the method for calculating s-layer removal and membrane ruffling that uses normal vectors from the inner membrane to calculate membrane separation and the presence or absence of S-layer. See text for more details. (B) Percentage of cell surface covered by S-layer for each time point (colored bars). Circles show single-cell measurements and error bars represent the standard error of the mean of single-cell measurements. (C) Same as B, except quantification of membrane ruffling. See text for further details.