FIG. 1.

Schematic representation of the BglII D region of the KOS genome. The BglII D (25.9-kb) fragment encodes UL31 to UL38 (29). The direction of the UL36 transcript is indicated below the ORF. The UL36 ORF starts at genome nucleotide 80543 and stops at 71051. Three cell lines that express UL36 were made: B80, which was transformed with the BglII D fragment (pKBGD); XS13, transformed with the XbaI-to-SnaB1 fragment (pKXSB); and HS30, transformed with the HpaI-to-SnaB1 fragment containing a deletion in the UL35 gene (pKUL36). The deletion in pKΔUL36 spans from the KpnI to EcoRV sites in UL36. Relevant restriction enzyme sites and genome nucleotide numbers (29) in parentheses are indicated at the top.