FIG. 2.

Infected cell polypeptide synthesis in KΔUL36-infected cells. Vero cells (10⁶ in 35-mm-diameter dishes) were infected with KOS (lane 2) and KΔUL36 (lane 3) at an MOI of 10 PFU/cell or mock infected (lane 1). The infected cells were metabolically labeled with [³⁵S]methionine from 9 to 24 h postinfection. Cells were solubilized in Laemmli sample buffer, and the proteins were analyzed by SDS-PAGE (9% acrylamide). Protein standards (lane M) correspond to 220, 97.4, 66, and 46 kDa (the 97.4-kDa marker migrates as a doublet in our gels). The closed circle indicates the position of the UL36 protein in KOS lysates.