FIG. 3.

Cleavage of viral genomic DNA in KΔUL36-infected cells. Vero (lanes 1 and 2) and HS30 (lanes 3 and 4) cells (10⁶ in 35-mm-diameter dishes) were infected with KOS (lanes 1 and 3) and KΔUL36 (lanes 2 and 4) at an MOI of 10 PFU/cell. Infected cell DNA was prepared 24 h postinfection, and 2 μg was digested with BamHI. The restriction fragments were analyzed by Southern blot hybridization using a ³²P-labeled DNA fragment corresponding to the BamHI K junction fragment. The BamHI K junction fragment and terminal Q and S fragments are indicated at the right; shown below the blot is a schematic representation of the HSV-1 genome and locations of the BamHI junction and terminal fragments.