FIG. 4.

Capsid formation in KΔUL36-infected cells. Vero cell monolayers (10⁷ cells) were infected with KOS (A) and KΔUL36 (B) at an MOI of 10 PFU/cell and labeled with [³⁵S]methionine from 8 to 24 h postinfection. Nuclear extracts were prepared and layered onto 20 to 50% sucrose gradients. Fractions collected after sedimentation were TCA precipitated, and the proteins in the fractions were resolved by SDS-PAGE (17% acrylamide). Direction of sedimentation was from right to left. The positions of capsid proteins are indicated at the left for KOS; the positions at which A, B, and C capsids sediment are indicated at the bottom.