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Definition of the minimal domain of the CSFV Alfort/187 E2 protein essential for binding by polyclonal IgG antibodies of an experimentally CSFV-infected pig serum. Deletion constructs for expression of targeted regions of E2 were generated as described in Materials and Methods. Reactivity of the fusion products with pig antiserum was analyzed by Western blotting. Symbols used here are explained in the Fig. 2 legend.
