TABLE 1.

List of primers^a used to amplify cDNA fragments coding for targeted regions of the CSFV E2 protein

Primer	Nucleotide sequence	Position (nucleotides)
PA	5′ GGATCCCAGCTAGCCTGCAAGGAAGA 3′	2441 to 2460
PB	5′ AAGCTTGGGTAGTGTGGGAGTCCGTC 3′	3083 to 3102
P3	5′ GTGGAATTCGAGCTCCTGTTCGACGGGAC 3′	2681 to 2700
P4	5′ ACAGTCGACTCTGTTCTCAGAGTTGTTGGG 3′	2869 to 2889
P5	5′ GTGGAATTCGTGGTAAAGACCTTCAGGAG 3′	2891 to 2910
P6	5′ ACAGTCGACGGGTAGTGTGGGAGTCCGTC 3′	3083 to 3102
P7	5′ GTGGAATTCTACAATACAACCTTGTTGAAC 3′	2783 to 2803
P8	5′ ACAGTCGACTTGCCCCCCAACTTACAGTAG 3′	2974 to 2994
P9	5′ GTGGAATTCCAGCTAGCCTGCAAGGAAGAT 3′	2441 to 2461
P10	5′ GTGGAATTCGACGGGACCGTTAAGGCCATT 3′	2561 to 2581
P11	5′ ACAGTCGACCCGTTCAACAAGGTTGTATTG 3′	2785 to 2805
P14	5′ ACAGAATTCGGGTGGACGGGTGTTATAGAG 3′	2834 to 2854
P15	5′ GTGGTCGACCAATCCATTCTGTGCGGAAAG 3′	2920 to 2940
P33	5′ GTGGAATTCAGCCCAACAACTCTGAGAACAG 3′	2867 to 2888
P44	5′ ACAGAATTCACGGGTGTTATAGAGTGCACAGC 3′	2840 to 2862
P45	5′ GTGGAATTCGTTATAGAGTGCACAGCAGTG 3′	2846 to 2866
P46	5′ ACAGAATTCGAGTGCACAGCAGTGAGCCCA 3′	2852 to 2872
P47	5′ ACAGAATTCACAGCAGTGAGCCCAACAACTC 3′	2858 to 2879
P48	5′ ACAGTCGACCTCAGAGTTGTTGGGCTCACTGC 3′	2861 to 2883
P49	5′ ACAGTCGACACCACTTCTGTTCTCAGAGTTG 3′	2874 to 2895

^aPrimers are derived from the nucleotide sequence of the CSFV strain Alfort/187 genome (27) (GenBank accession number X87939). Restriction sites (BamHI, HindIII, EcoRI, and SalI) introduced at 5′ ends are underlined.