Fig. 4.

Assessment of ROS production in differentiated SH-SY5Y cells treated with Aβ1-42, MOL, and MOL with Aβ1-42. ROS production was evaluated with DHE staining. (A) Control; (B) Aβ42-1 treatment alone; (C) Aβ1-42 treatment alone; (D) 100-μg/mL MOL alone; (E-G) 25, 50, and 100-μg/mL MOL pretreatment followed by Aβ1-42 exposure. Bar = 100 μm. (G) Quantification of fluorescent DHE intensity. Data are expressed as mean ± SEM from 3 independent experiments. ^**P < 0.01 indicates a significant difference in comparison with day 0 which was considered the control group; ^#P < 0.05 and ^##P < 0.01 imply the differences between designated groups. Aβ, Amyloid beta; MOL, Moringa oleifera leaf; DHE, dihydroethidium; ROS, reactive oxygen species.