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. 2024 Feb 6;19(1):105–120. doi: 10.4103/1735-5362.394825

Fig. 5.

Fig. 5.

Assessment of apoptotic indicators in differentiated SH-SY5Y cells treated with Aβ1-42, MOL, and MOL with Aβ1-42. (A and B) Immunoblots of Bax and Bcl-2, respectively. Band quantification was obtained from 3 independent experiments. The density of the bands was normalized with that of β-actin data are expressed as mean ± SEM of percentage to the control. (C) Immunofluorescence staining with Hoechst33258 nuclear staining (blue) and caspase-3/7 (green); Bar = 50 μm; imaging results are representative of 3 independent experiments. (D) Quantification of mean fluorescent intensity caspase-3/7 positive cells; data are expressed as mean ± SD. (E) Immunoblots of caspase-3. (F and G) Band quantification of cleaved caspase-3 and pro-caspase-3, respectively, was obtained from 3 independent experiments; the density of the bands was normalized with that of β-actin data are expressed as mean ± SEM of percentage to the control. *P < 0.05 indicates a significant difference in comparison with day 0 which was considered the control group; #P < 0.05, ##P < 0.01 and ###P < 0.001 imply the differences between designated groups. Aβ, Amyloid beta; MOL, Moringa oleifera leaf; DHE, dihydroethidium; Bax, B-associated X protein; Bcl-2, B-cell lymphoma-2.