Fig. 6.

Effects of MOL pretreatment on cell apoptosis in Aβ1-42-treated SH-SY5Y cells. Cells were stained with Hoechst33258. (A) Control; (B) Aβ42-1 treatment alone; (C) Aβ1-42 treatment alone; (D) 100-μg/mL MOL alone; (E-G) 25, 50, and 100-μg/mL MOL pretreatment followed by Aβ1-42 exposure. Condensed or fragmented nuclei (a white arrow) were considered apoptotic nuclei. (H) Quantification of apoptotic nuclei; data are expressed as mean ± SEM from 3 independent experiments. ^***P < 0.001 indicates a significant difference in comparison with day 0 which was considered the control group; ^#P < 0.05 and ^##P < 0.01 imply the differences between designated groups. Aβ, Amyloid beta; MOL, Moringa oleifera leaf.