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. 2024 Feb 6;19(1):93–104. doi: 10.4103/1735-5362.394824

Fig. 2.

Fig. 2.

Different conditions of total expression of recombinant proteins. (A) Recombinant LYC-I peptide in fusion to inteins 1 and 2. Lane 1, unstained protein ladder 26614 PageRuler™(Thermoscietific, USA); lane 2, total E. coli proteins induced by 0.1 mM IPTG at 7 °C; lane 3, total E. coli proteins induced by 0.3 mM IPTG at 7 °C; lane 4, total E. coli proteins induced by 0.5 mM IPTG at 7 °C; lane 5, total E. coli proteins induced by 0.1 mM IPTG at 22 °C; lane 6, total E. coli proteins induced by 0.3 mM IPTG at 22 °C; lane 7, total E. coli proteins induced by 0.5 mM IPTG at 22 °C; lane 8, total E. coli proteins induced by 0.1 mM IPTG at 37 °C; lane 9, total E. coli proteins induced by 0.3 mM IPTG at 37 °C; lane 10, total E. coli proteins induced by 0.5 mM IPTG at 37 °C; (B) recombinant DFF40-LYC-I in fusion to inteins 1 and 2. Lanes 1 and 8, unstained protein ladder 26614 PageRuler™; lane 2, total E. coli proteins induced by 0.1 mM IPTG at 22 °C; lane 3, total E. coli proteins induced by 0.3 mM IPTG at 22 °C; lane 4, total E. coli proteins induced by 0.5 mM IPTG at 22 °C; lane 5, total E. coli proteins induced by 0.1 mM IPTG at 7 °C; lane 6, total E. coli proteins induced by 0.3 mM IPTG at 7 °C; lane 7, total E. coli proteins induced by 0.5 mM IPTG at 7 °C; lane 9, total E. coli proteins induced by 0.1 mM IPTG at 37 °C; lane 10, total E. coli proteins induced by 0.3 mM IPTG at 37 °C; lane 11, total E. coli proteins induced by 0.5 mM IPTG at 37 °C. LYC-I, lycosin-I; E. coli, Escherichia coli; IPTG, isopropyl β-d-1-thiogalactopyranoside; DFF, DNA fragmentation factor.