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. 2024 Feb 6;19(1):93–104. doi: 10.4103/1735-5362.394824

Fig. 3.

Fig. 3.

Different conditions of the soluble expression of recombinant proteins. (A). LYC-I peptide in fusion to inteins 1 and 2. Lane 1, unstained protein ladder 26614 PageRuler™; Lane 2, expression of E. coli protein solution induced by 0.1 mM IPTG at 7 °C; Lane 3, expression of E. coli protein solution induced by 0.3 mM IPTG at 7 °C; Lane 4, expression of E. coli protein solution induced by 0.5 mM IPTG at 7 °C; lane 5, expression of E. coli protein solution induced by 0.1 mM IPTG at 22 °C; lane 6, expression of E. coli protein solution induced by 0.3 mM IPTG at 22 °C; lane 7, expression of E. coli protein solution induced by 0.5 mM IPTG at 22 °C; lane 8, expression of E. coli protein solution induced by 0.1mM IPTG at 37 °C; lane 9, expression of E. coli protein solution induced by 0.3 mM IPTG at 37 °C; lane 10, expression of E. coli protein solution induced by 0.5 mM IPTG at 37 °C; (B) DFF40-LYC-I in fusion to inteins 1 and 2. Lanes 1 and 8, unstained protein ladder 26614 PageRuler™; lane 2, expression of E. coli proteins induced by 0.1 mM IPTG at 7 °C; lane 3, expression of E. coli proteins induced by 0.3 mM IPTG at 7 °C; lane 4, expression of E. coli proteins induced by 0.5 mM IPTG at 7 °C; lane 5, expression of E. coli proteins induced by 0.1 mM IPTG at 15 °C; lane 6, expression of E. coli proteins induced by 0.3 mM IPTG at 15 °C; lane 7, expression of E. coli proteins induced by 0.5 mM IPTG at 15 °C; lane 9 expression of E. coli proteins induced by 0.1 mM IPTG at 37 °C; lane 10, expression of E. coli proteins induced by 0.3 mM IPTG at 37 °C; lane 11, expression of E. coli proteins induced by 0.5 mM IPTG at 37 °C. LYC-I, lycosin-I; E. coli, Escherichia coli; IPTG, isopropyl β-d-1-thiogalactopyranoside; DFF, DNA fragmentation factor.