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Purification of peptide and protein using the IMPACT system. (A). Purified LYC-I. Lane 1, low range unstained protein ladder 26635 (Thermoscientif, USA); lane 2, elution of LYC-I removed from the column; (B) purified DFF40-LYC-I. Lane 1, unstained protein ladder 26612 (Thermoscientif, USA); lane 2, elution of DFF40-LYC-I removed from the column. IMPACT, intein mediated purification with an affinity chitin-binding tag; LYC-I, lycosin-I; DFF, DNA fragmentation factor.
