Fig 1. Comparison of Norwegian isolates seeding activity in RT-QuIC.
Ten percent brain homogenates were subjected to serial dilutions from 2 x 10−1 to 2 x 10−6 in RT-QuIC seed dilution buffer. Samples were considered positive when a minimum of two out of four wells crossed the threshold relative fluorescence unit (RFU). Threshold is the average RFU of all negative control reactions plus five times their standard deviation. Negative control was a CWD-negative mule deer. R-NO16 and M-NO3 were positive for prion seeding activity up to 2 x 10−4 dilution (upper panels), and H-NO1 up to 2 x 10−3 dilution (bottom left panel). The y-axis represents the RFU, and the x-axis represents time in hours (h). Mouse recombinant PrP was used as substrate for the RT-QuIC reactions.