Fig 6. PrPres detection in brain homogenates of TgElk mice inoculated with H-NO1.
Ten percent brain homogenates were digested with 50 μg/mL PK for one hour at 37°C. Anti-PrP antibodies used were 4H11 (1:500, upper panel) and 12B2 (1:1000, lower panel). Lanes 1–4 corresponds to TgElk mice inoculated i.c. with the H-NO1 brain homogenate. Lanes 5–9 correspond to TgElk mice inoculated with brain homogenate from the TgElk in lane 4. pos = PrPres-positive TgElk control; neg = non-inoculated TgElk; kDa = kilodalton.