Table 2. Number of mouse brains and spleens positive for PrPres on WB and/or prion seeding activity in RT-QuIC per total number of samples tested within each respective group.
| Number of positive samples / number of samples tested | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Prnp.Cer.Wt | Prnp.Cer.138NN | |||||||||||||||
| Brain | Spleen | Brain | Spleen | |||||||||||||
| Inoculum | Tissue | roi | WB | RT-QuIC | RT-QuIC | WB | RT-QuIC | RT-QuIC | ||||||||
| PrPres | -1 | -2 | -3 | -1 | -2 | -3 | PrPres | -1 | -2 | -3 | -1 | -2 | -3 | |||
| R-NO16 | Brain | i.c. | 5/7 | – | – | – | 2/7 | 3/7 | 3/7 | 0/4 | 0/4 | 2/4 | 2/4 | 0/4 | 0/4 | 0/4 |
| Reindeer | ||||||||||||||||
| i.p. | 6/7 | – | – | – | 2/6 | 4/6 | 4/6 | 0/3 | 1/3 | 1/3 | 0/3 | 2/3 | 2/3 | 2/3 | ||
| M-NO3 | Brain | i.c. | 4/6 | – | – | – | 0/5 | 0/5 | 0/5 | 2/5 | 2/5 | 3/5 | 1/5 | 0/5 | 0/5 | 0/5 |
| Moose | ||||||||||||||||
| i.p. | 0/5 | 0/5 | 0/5 | 0/5 | 0/4 | 0/4 | 0/4 | 0/5 | 2/5 | 4/5 | 1/5 | 0/4 | 0/4 | 0/4 | ||
| H-NO1 | Brain | i.c. | 0/5 | 0/5 | 5/5 | 0/5 | 0/3 | 0/3 | 1/3 | 0/4 | 0/4 | 3/4 | 2/4 | 0/4 | 0/4 | 0/4 |
| Red deer | ||||||||||||||||
| i.p. | na | na | na | na | na | na | na | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | ||
The numbers -1, -2, and -3 refer to 2 x 10−1, 2 x 10−2, and 2 x 10−3 RT-QuIC seed dilutions, respectively. Samples were considered positive in RT-QuIC when a minimum of two out of four replicates crossed the threshold relative fluorescence unit (RFU, see methods). Brain homogenates positive for PrPres on WB (Fig 2) were not tested in RT-QuIC, except for Prnp.Cer.138NN mouse brains inoculated i.c. with M-NO3. Brain homogenates from Prnp.Cer.Wt mice inoculated i.c. and i.p. with R-NO16 and i.c. with M-NO3 that were negative for PrPres on WB (Fig 2) were tested in RT-QuIC but not included in this table (data shown in Fig B in S1 Text). roi = route of inoculation; WB = western blot; i.c. = intracerebral; i.p. = intraperitoneal;– = not tested; na = not inoculated.