(A) Subgenomic RNA quantification of the N gene (sgN) of SARS-CoV-2 by RT-qPCR in copies/mL from bronchoalveolar lavage (BAL), nasal swabs, and throat swabs. (B) sgN copies/gram of tissue at necropsy (day 28–35 post-infection) from spleen, axillary lymph node (axLN), non-PET/CT avid pulmonary lymph nodes (norm. pLN), previously PET/CT hot pulmonary lymph nodes (prev. hot pLN), salivary gland (SG), tonsil, nasal turbinates (nasal turb.), normal lung sections (norm. lung), and previously PET/CT hot lung sections. For A the cutoff for RNA detection is 3,000 copies/mL. For B the cutoff is 2,000 copies/gram of tissue. Graphs show individual animals from samples taken at baseline, days 2, 3, 6, 7, 10, 14, and 22 post-infection, as well as necropsy. Significance calculated with a 2-way ANOVA and a Dunnett’s multiple comparison test.