|
Antibodies
|
| Rabbit Anti-cFOS |
CST |
CAT # 2250S |
| Mouse Anti-cFOS |
Santa Cruz |
CAT # sc-166940 |
| Rabbit Anti-Calb2 |
Abclonal |
CAT # A9807 |
| Donkey anti-mouse IgG-Alexa 594 |
Abcam |
CAT # ab150108 |
| Donkey anti-rabbit IgG-Alexa 647 |
Abcam |
CAT # ab150075 |
|
Bacterial and virus strains
|
| AAV9-hSyn-GCaMP6f |
Taitool Bioscience |
Cat# SO224-9-H20 |
| AAV9-hSyn-mCherry |
Vigene Technology |
N/A |
| AAV9-hSyn-ChR2-mCherry |
Vigene Technology |
N/A |
| AAV9-TRE3G-mCherry |
BrainVTA |
N/A |
| AAV9-TRE3G-ChR2-mCherry |
BrainVTA |
N/A |
| AAV2/9-TRE3G-NpHR-EYFP |
BrainVTA |
N/A |
| AAV-cfos-tTA-TRE3G-mCherry |
BrainVTA |
N/A |
| AAV-TRE3G-hM3D(Gq)-mCherry |
BrainVTA |
N/A |
| AAV2/retro-hSyn-EGFP |
BrainVTA |
N/A |
| AAV2/5-hSyn-DIO-hM3D(Gq)-EGFP |
BrainVTA |
N/A |
| AAV2/5-hSyn-DIO-hM4D(Gi)-EGFP |
BrainVTA |
N/A |
|
Chemicals
|
| Clozapine-N-oxide |
Tocris |
CAT # 4936/50 |
| DAPI |
Cell Signaling Technology |
CAT # 4083S |
| 4-Hydroxytamoxifen (4-OHT) |
Sigma |
CAT # H6278 |
| Experimental models: Organisms/strains |
| Mouse: C57BL/6N |
Department of Laboratory Animal Sciences, Peking University Health Science Center |
JAX 000664 |
| Mouse: cfos-tTA |
The Jackson Laboratory |
JAX 018306 |
| Mouse: Fos-CreERT2
|
The Jackson Laboratory |
JAX 021882 |
| Mouse: Ai9 |
The Jackson Laboratory |
JAX 007905 |
|
Software and algorithms
|
| Fiji (ImageJ) |
NIH |
https://fiji.sc/
|
| GraphPad Prism 9 |
GraphPad |
https://www.graphpad.com/scientificsoftware/prism/
|
| MATLAB |
MathWorks |
https://www.mathworks.com
|
| Suite2p |
HHMI Janelia Research Campus |
https://www.suite2p.org/
|
