|
Antibodies
|
| Goat anti-Mouse/Rat CD31/PECAM-1 antibody |
R&D Systems |
Cat# 3628; RRID: AB 2161028 |
| Rat anti-CD31 antibody |
Abcam |
Cat# ab7388; RRID: AB 305905 |
| PE Rat Anti-Mouse CD31 |
BD Biosciences |
Cat# 553373; RRID: AB 394815 |
| PerCP Rat Anti-Mouse CD45 |
BD Biosciences |
Cat# 557235; RRID: AB 10642171 |
| Mouse (IgG2b) Myosin Heavy Chain Type I antibody (BA-F8) |
DSHB |
Cat# BA-F8; RRID: AB 10572253 |
| Mouse (IgG1) Myosin Heavy Chain Type IIA antibody (SC-71) |
DSHB |
Cat# SC-71; RRID: AB 2147165 |
| Mouse (IgM) Myosin Heavy Chain Type IIB antibody (BF-F3) |
DSHB |
Cat# BF-F3; RRID: AB 2266724 |
| Total OXPHOS Rodent WB Antibody Cocktail |
Abcam |
Cat# ab110413; RRID: AB 2629281 |
| PE Hamster Anti-Mouse CD36 |
BioLegend |
Cat# 102605; RRID: AB 389349 |
| Rabbit anti-CD36 antibody |
Abcam |
Cat# ab124515; RRID: AB 2924667 |
| Rabbit anti-PDK4 antibody |
Abcam |
Cat# ab214938; RRID: AB 2864318 |
| Goat anti Human/Mouse Lipoprotein lipase antibody |
R&D Systems |
Cat# AF7197; RRID: AB 10972480 |
| Wheat Germ Agglutinin (WGA) |
Thermo Fisher Scientific |
Cat# W11262
|
| IRDye 800RD Goat anti-Mouse IgG (H+L) |
LI-COR BioScience |
Cat# LIC-926–68070 |
| IRDye 680RD Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed |
LI-COR BioScience |
Cat# LIC-926–68071 |
| Anti-rabbit IgG, HRP-linked |
Cell Signaling |
Cat# 7074 |
| Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 |
Thermo Fisher Scientific |
Cat# A-21141 |
| Goat anti-Mouse IgG1 Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 350 |
Thermo Fisher Scientific |
Cat# A-211120 |
| Goat anti-Mouse IgGM (Heavy Chain) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 |
Thermo Fisher Scientific |
Cat# A-21043 |
|
Chemicals, peptides, and recombinant proteins
|
| Collagenase IV |
Thermo Fisher Scientific |
Cat# 17104019 |
| Dispase II |
Sigma-Aldrich |
Cat# D4693 |
| Hoechst |
Thermo Fisher Scientific |
Cat# 62249 |
| 5-Ethynyl-2’-deoxyuridine (EdU) |
Thermo Fisher Scientific |
Cat# C10632/4 |
| Alexa-647 Fluor conjugated isolectin B4 |
Thermo Fisher Scientific |
Cat# I32450
|
| Tamoxifen |
Sigma-Aldrich |
Cat# T5648 |
| Axitinib |
MedChemExpress |
Cat# HY-10065 |
| InVivoMAb rat IgG1 isotype control |
BIOZOL |
Cat# BXC-BE0088 |
| InVivo MAb anti Mouse VEGFR- 2, Clone: [DC101], Rat, Monoclonal |
BIOZOL |
Cat# BXC-BE0060 |
| Linoleic Acid (18:2), sodium salt (U-13C18, 98%) |
Cambridge Isotope Laboratories |
Cat# CLM-10487-PK |
| Palmitic Acid, [9,10-3H(N)]-, 1mCi, (37MBq) |
Perkin Elmer |
Cat# NET043001MC |
| BODIPY™ 493/503 |
Thermo Fisher Scientific |
Cat# D2191 |
| mouse recombinant FGF21 |
Preprotech |
Cat# 450–56 |
|
Critical commercial assays
|
| Pierce BCA Protein Assay Kit |
Thermo Fisher Scientific |
Cat# 23225 |
| mVEGF Elisa |
R&D Systems |
Cat# MMV00 |
| mVEGFB Elisa |
Abcam |
Cat# ab289700 |
| mFGF21 Elisa |
R&D Systems |
Cat# MF2100 |
| RNeasy Kit |
QIAGEN |
Cat# 74034 |
| High Capacity cDNA Reverse Transcription Kit |
Thermo Fisher Scientific |
Cat# 43–688-13 |
| SYBRGreen-based Master Mix |
Thermo Fisher Scientific |
Cat# A25778 |
| Click-iT Cell Reaction Buffer Kit |
Thermo Fisher Scientific |
Cat# C10269
|
|
Deposited data
|
| Muscle transcriptomics raw and analyzed |
This paper |
Table S2 and 6
|
| Muscle lipidomics |
This paper |
Table S3
|
| Muscle and plasma metabolomics from WT and WT and CD36EC−/− mice |
This paper |
Table S4–5
|
|
Experimental models: Organisms/strains
|
| Mouse: C57BL/6J wild type |
Charles River Laboratories |
N/A |
| Mouse: CD36fl/fl
|
Charles River Laboratories |
N/A |
| Mouse: fgf21fl/fl
|
Charles River Laboratories |
N/A |
| Mouse: pdgfb-CreERT2
|
(Claxton et al., 2008) |
N/A |
|
Oligonucleotides
|
|
Fgf21: F: CAAATCCTGGGTGTCAAAGC |
N/A |
N/A |
|
Fgf21: R: CATGGGCTTCAGACTGGTAC |
N/A |
N/A |
|
Ucp1: F: GCATTCAGAGGCAAATCAGC |
N/A |
N/A |
|
Ucp1: R: GCCACACCTCCAGTCATTAAG |
N/A |
N/A |
|
18s: F CATGCAGAACCCACGACAGTA
|
N/A |
N/A |
|
18s: R CCTCACGCAGCTTGTTGTCTA
|
N/A |
N/A |
|
Actin: F AGCTTCTTTGCAGCTCCTTCGTTG
|
N/A |
N/A |
|
Actin: R TTCTGACCCATTCCCACCATCACA
|
N/A |
N/A |
|
Software and algorithms
|
| FlowJo Software (version 10.4.2) |
Three Star |
https://www.flowjo.com/
|
| ImageJ (for image analysis) |
NIH |
https://imagej.nih.gov/ij/
|
| Prism 8 (version 8.0.0) |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/
|
| Adobe Illustrator CS6 (version 16.0.4) |
Adobe |
https://www.adobe.com/
|
| R 4.0.3 |
cran.r-project |
https://cran.r-project.org/bin/windows/base/
|
| El Maven |
Elucidata |
https://github.com/ElucidataInc/ElMaven/releases
|
