Fig. 5 ∣. Limit of detection analysis.

Fluorescence values at 150 minutes from NASBA-Cas13a with varying concentrations of synthetic SARS-CoV-2 genome using a, gRNA 2–1, b, gRNA 7–1, c, gRNA 7–2, or d, gRNA 8–1. Data are n=3 independent biological replicates, each plotted as a point with raw fluorescence values standardized to MEF. Bar height represents the average of the replicates. Error bars indicate the average of the replicates ± standard deviation. Input RNA concentrations for which signal is distinguishable from background (without input RNA) were determined using a two-sided, heteroscedastic Student’s t-test. ***P < 0.001, **P = 0.001–0.01, *P = 0.01–0.05). P values and degrees of freedom are listed in Supplementary Data 3.