Table 1.
Characteristics of Perturb-Seq datasets analyzed.
| Dataset (Technology) |
Reference | Cell type |
Perturbed Gene-Set |
Number of perturbations |
Median # cells per perturbation |
|---|---|---|---|---|---|
| K562-GenomeWide (CRISPRi) | Replogle et al, 2022, | K562 | All expressed genes | 9866 genes | 178 |
| K562-Essential (CRISPRi) | Replogle et al, 2022 | K562 | Essential genes | 2057 | 121 |
| RPE1-Essential (CRISPRi) | Replogle et al, 2022 | RPE1 | Essential genes | 2393 | 72 |
| Jurkat-Essential (CRISPRi) | Novel | Jurkat | Essential genes | 2393 | 83 |
| HepG2-Essential (CRISPRi) | Novel | HepG2 | Essential genes | 2393 | 45 |
| K562-Titration (CRISPRi) | Jost et al, 2020 | K562 | Essential genes | 25 genes, 128 guides | 143 |
| Sox9-Titration (dTAG) | Naqvi et al, 2023 | iCNCC | SOX9 | 5 dTAG degron concentrations | 7 bulk samples per concentration |
| Polycomb-Titration (dTAG) | Weber et al, 2021 | mESC | Ring1b, EED (Simultaneous) | 4 dTAG degron concentrations | 4 bulk samples per concentration |