Figure 1. Sleep, feeding, and post-prandial sleep behaviors across three populations of wild-type Astyanax mexicanus.

A) 20 dpf fish were briefly fed prior to 24 h behavioral sleep recordings. At ZT0 the following day, fish were assayed for feeding behavior until ZT2, immediately after which we recorded sleep behaviors between ZT2 and 6. B) Sleep profiles of wild type surface, Pachón, and Tinaja fish taken over the experiment. Lines and error bars represent the mean ± SD. C) Cross-population comparison of total sleep duration immediately following the feeding experiment. Cavefish slept significantly less than surface fish (ANOVA: F_{2, 34} = 8.123, p = 0.0013; Tukey’s HSD for surface-Pachón, p = 0.0202, p = 0.0024; Tukey’s HSD for surface-Tinaja, p = 0.0024). D) Cross-population comparison of the number of Artemia eaten during the two-h feeding experiment. Tinaja ate significantly more than surface fish (ANOVA: F_{2, 76} = 3.91, p = 0.0242; Tukey’s HSD for surface-Tinaja, p = 0.0178).