Table 2.

Summary of predictive CpGs of DNA methylation-based clocks, biomarker predictors, and cell type deconvolution in EPICv1 and EPICv2.

Predictors	CpGs	Probes absent in EPICv1 (%)	Probes absent in EPICv2 (%)	Probes absent in EPICv1 and reintroduced in EPICv2	EPICv2 replicate probes	Training arrays; training age range (year)	Training sample size	Probes absent in Infinium Methylation Screening Array (MSA)	Probes absent in 450K
Horvath pan-tissue*	353	17 (4.82%)	13 (3.68%)	14	4(1.13%)	27K and 450K Age: 0–100	7844	6(1.7%)	0
Hannum*	71	6 (8.45%)	7 (9.86%)	4	5(7.04%)	450K Age: 19–101	656	3(4.23%)	0
Horvath SkinBlood	391	0	17 (4.35%)	0	9(2.3%)	450K and EPICv1 Age: 0–94	896	3(0.77%)	0
PhenoAge	513	0	18 (3.51%)	0	7(1.36%)	27K, 450K, EPICv1 Age: 18–100	9926	3(0.58%)	0
GrimAge**	1030	na	na	0	na	450K and EPICv1 Age: NA (mean 66)	1731	NA	na
PC Clocks***	78464	0	5801 (7.39%)	0	650 (0.83%)	450K and EPICv1 Age: 0–101	na	51108 (65.14%)	0
DunedinPACE	173	0	29 (16.76%)	0	2(1.16%)	EPICv1 Age 38 and 45	1037	2(1.16%)	0
DNAmTL	140	0	31 (22.14%)	0	3(2.14%)	450K and EPICv1 Age: 22–93	2256	2(1.43%)	0
epiTOC*	385	31 (8.05%)	26 (6.75%)	25	0	450K Age: 19–101	656	13(3.38%)	0
IL-6 score	35	0	3 (8.57%)	0	1(2.86%)	450K and EPICv1 Age: 67–78	875	12(34.29%)	0
CRP score*	1765	103 (5.84%)	96 (5.44%)	83	41 (2.32%)	27K, 450K and EPICv1 Age: 16–75	22774	357 (20.23%)	0
Smoking score	233	0	23 (9.87%)	0	2(0.86%)	EPICv1 Age: 18–99	5087	27(11.59%)	0
Alcohol score	450	0	49 (10.89%)	0	6(1.33%)	EPICv1 Age: 18–99	5087	47(10.44%)	0
IDOL	1200	0	8 (0.67%)	0	48(4%)	EPICv1 Age: 19–58	NA	20(1.67%)	741 (61.75%)

^*CpGs commonly absent in both EPICv1 and EPICv2: Horvath pan-tissue; 3 CpGs; Hannum; 2 CpGs; EpiTOC; 6 CpGs; CRP; 20 CpGs.

^**Clock CpGs for GrimAge are not publicly available.

^***PC Clocks CpGs include the total CpGs required to calculate PC versions of Horvath pan-tissue, Hannum, Horvath SkinBlood, PhenoAge, and GrimAge.