Table 2.

Summary of predictive CpGs of DNA methylation-based clocks, biomarker predictors, and cell type deconvolution in EPICv1, EPICv2, Infinium Methylation Screening Array (MSA), and 450K.

Tools	CpGs	EPICv1 absent CpGs	EPICv2 absent CpGs	EPICv2 replicate probes	MSA absent CpGs	450K absent CpGs	Training arrays (sample size, age in years)
Horvath pan-tissue*	353	19 (5.38%)	13 (3.68%)	4 (1.13%)	6 (1.7%)	0	27K, 450K (n=7844, 0–100)
Hannum*	71	6 (8.45%)	7 (9.86%)	5 (7.04%)	3 (4.23%)	0	450K (n=656, 9–101)
Horvath Skin and Blood	391	0	17 (4.35%)	9 (2.3%)	3 (0.77%)	0	450K, EPICv1 (n=896, 0–94)
PhenoAge	513	0	18 (3.51%)	7 (1.36%)	3 (0.58%)	0	27K, 450K, EPICv1 (n=9926, 18–100)
GrimAge**	1030	NA	NA	NA	NA	NA	450K, EPICv1 (n=1731, mean 66)
PC Clocks***	78464	0	5801 (7.39%)	650 (0.83%)	51108 (65.14%)	0	450K, EPICv1 (n=NA, 0–101)
DunedinPACE	173	0	29 (16.76%)	2 (1.16%)	2 (1.16%)	0	EPICv1 (n=1037, 38 and 45)
DNAmTL	140	0	31 (22.14%)	3 (2.14%)	2 (1.43%)	0	450K, EPICv1 (n=2256, 22–93)
epiTOC*	385	31 (8.05%)	26 (6.75%)	0	13 (3.38%)	0	450K (n=656, 19–101)
IL-6 score	35	0	3 (8.57%)	1 (2.86%)	12 (34.29%)	0	450K, EPICv1 (n=875, 67–78)
CRP score*	1765	104 (5.89%)	96 (5.44%)	41 (2.32%)	357 (20.23%)	0	27K, 450K, EPICv1 (n=22774, 16–75)
Smoking score	233	0	23 (9.87%)	2 (0.86%)	27 (11.59%)	0	EPICv1 (n=5087, 18–99)
Alcohol score	450	0	49 (10.89%)	6 (1.33%)	47 (10.44%)	0	EPICv1 (n=5087, 18–99)
IDOL	1200	0	8 (0.67%)	48 (4%)	20 (1.67%)	741 (61.75%)	EPICv1 (n=56, 19–58)

^*There are CpGs commonly absent in both EPICv1 and EPICv2 in these clocks: Horvath pan-tissue: 3 CpGs; Hannum: 2 CpGs; epiTOC: 6 CpGs; CRP score: 20 CpGs. There are probes absent in EPICv1 and are reintroduced in EPICv2 in these clocks: Horvath pan-tissue: 14 CpGs; Hannum: 4 CpGs; epiTOC: 25 CpGs; CRP score: 83 CpGs.

^**Clock CpGs for GrimAge are not publicly available.

^***PC Clocks CpGs include the total CpGs required to calculate PC versions of Horvath pan-tissue, Hannum, Horvath SkinBlood, PhenoAge, and GrimAge.