Figure 2. Y404W mutant adopts an open conformation in the absence of ligands.

(a) Structural comparison between PI(3,5)P₂/Tem-bound open structure (green) and the Y404W mutant structure (orange). Only the front subunit and the neighboring S1-S4 regions are highlighted in color for clarity. (b) Zoomed-in views of the regions surrounding Y404 (WT, green) and W404 (mutant, orange).