Step #	Temperature	Time (minute : seconds)
1	95°C	15 : 00
2	94°C	0 : 10
3	62°C	0 : 20
4	72°C	0 : 30
5	Plate read
6	Repeat steps 2 through 4 thirty nine (39) times
7	72°C	5 : 00
8	Melting Curve from 72°C to 95°C Read every 0.2°C, hold for 00.01
10	4°C	Hold until plate retrieved