Figure 8. Shared patterns in transcript rhythmicity across cell types in females.

(A) Threshold free approach (rank-rank hypergeometric overlap) assessing the overlap of rhythmic transcripts between cell types in females. There is moderate overlap in rhythmic transcripts between cell types in females. (B) Pathway analysis (IPA) of rhythmic transcripts (p<0.05) across cell types in females. The top 10 pathways and their overlap between cell types are plotted. Pathways enriched for rhythmic transcripts show substantial overlap between cell types particularly in pathways associated with cancer. (C) The top 10 pathways (IPA) associated with transcripts that are uniquely rhythmic in pyramidal cells (top) or PV cells (bottom). Uniquely rhythmic transcripts in pyramidal cells are associated with a variety of processes including cytokine signaling, autophagy, and DNA repair while uniquely rhythmic transcripts in PV cells are largely associated with intercellular communication. (D) Radar plot comparing the peak times (ZT) of rhythmic transcripts between cell types across 24 hours. Data is presented as the percentage of total rhythmic transcripts peaking within each 2-hour bin. In both cell types, females show two distinct groups of transcripts, one peaking in the light phase and the other during the dark phase, with a phase shift of 2–4 hours between cell types. (E) Phase concordance plots showing the peak time (ZT) of individual shared rhythmic transcripts in PV cells (X axis) and pyramidal cells (Y axis). Shared rhythmic transcripts tend to peak around the same time between cell types. IPA=Ingenuity Pathway Analysis, PV=parvalbumin cells, PYR=pyramidal cells, ZT=zeitgeber time