Figure 2. Cryo-EM single particle analysis of 50 nm-GEMs.
A) High resolution map of the GEM encapsulin cage (left) and low-pass filtered GEM particle with GFP density (green, right) reveal a GEM with a diameter of 50 nm (scale bar right). B) 2D projection of Quasibacillus thermotolerans two-component encapsulin-based iron storage compartment (EMDB:9383, top) and the 50 nm-GEM (this study, bottom) shows a clear density outside of the encapsulin cage ascribable to GFP.
