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. Author manuscript; available in PMC: 2024 Jul 12.
Published in final edited form as: ACS Synth Biol. 2012 Nov 14;2(1):47–58. doi: 10.1021/sb300091d

Figure 1. Coupling 1-butanol-induced TetA expression to E. coli growth rate.

Figure 1

E. coli DH1 ΔadhE harboring tetA-gfp under control of PBMO displayed butanol-dependent changes in growth phenotype. (a) In positive selection mode, tetracycline (Tet) supplementation resulted in a positive correlation between E. coli growth rate and exogenous 1-butanol concentration. 1- butanol concentrations greater than 40 mM overwhelmed the synthetic selective pressure. (b) In negative selection mode, nickel-chloride (NiCl2) supplementation inverted the correlation between growth rate and exogenous 1-butanol concentration. Data are mean (s.d.) (n=4).