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. Author manuscript; available in PMC: 2024 Jul 12.
Published in final edited form as: ACS Synth Biol. 2012 Nov 14;2(1):47–58. doi: 10.1021/sb300091d

Figure 6. Demonstration of a synthetic selection for in vivo 1-butanol biosynthesis.

Figure 6

E. coli JAD#2 co-transformed with the pSelect#2, TetA-based synthetic selection device and either an RFP control plasmid (pRFPS2) or a non-functional alcohol biosynthetic pathway with a Z. mobilis pyruvate decarboxylase (pPDC) displayed poor growth upon addition of tetracycline selective pressure. Use of the L. lactis promiscuous 2-keto acid decarboxylase pathway (pKivD#2) or introduction of a single point mutation into the Z. mobilis pyruvate decarboxylase (pPDCI472A), imparting 2-oxobutanoate decarboxylase activity, resulted in improved fitness relative to the negative control strains. Data are mean (s.d.) (n=3).