Table 1.
| Strain | Genotype | Source |
|---|---|---|
| DH1 | endA1 recA1 thi-1 gyrA96 glnV44 relA1 hsdR17 (rk− mk+) λ− | ATCC |
| K12 | F’ proA+B+
lacIq ΔlacZM15/fhuA2 Δ(lac-proAB) glnV gal R(zgb-210::Tn10)TetS endA1 thi-1 Δ(hsdS-mcrB)5 |
NEB |
| DH1 ΔadhE | DH1 ΔadhE | This study |
| JAD-1 | DH1 ΔadhE (ilvD-ilvC)::neo | This study |
| JAD-2 | DH1 ΔadhE Δ(ilvD-ilvC) | This study |
| Plasmid | Description | Resistance | Origin | Source |
|---|---|---|---|---|
| pBMO#1 | PBmoR-bmoR, PBMO-gfp | CbR | ColE1 | This study |
| pSelect#1 | PBmoR-bmoR, PBMO-tetA-gfp | CbR | ColE1 | This study |
| pSelect#2 | PBmoR-bmoR, PBMO-tetA | CbR | ColE1 | This study |
| pC6Select#1 | PPcaR-pcaR | CmR | pSC101 | This study |
| pC6Select#2 | PpcaIJ-tetA | CbR | ColE1 | This study |
| pC4Select#1 | PdctA-tetA | CbR | ColE1 | This study |
| pKivD#1* | Ptrc-kivD.ADH6 | CmR | p15a | This study |
| pKivD#1-S71* | Ptrc-kivD.ADH6; optimized RBS | CmR | p15a | This study |
| pKivD#2 | PLacO1-kivD.ADH6 | CmR | pSC101 | This study |
| pPDC | PLacO1-PDC.ADH6 | CmR | pSC101 | This study |
| pPDC(I472A) | PLacO1-PDC(I472A).ADH6 | CmR | pSC101 | This study |
| pRFPA2 | Ptrc-rfp | CmR | p15a | 61 |
| pRFPS2 | PLacO1-rfp | CmR | pSC101 | 61 |