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Diagram of the parental bicistronic plasmid pT7CG. The vector is a derivative of pTM1 (19) and contains two reporter genes, CAT and GFP, that are separated by a short stretch of sequence (top) containing a unique AgeI site (underlined) used to insert ERAV sequences in frame with the GFP gene. The locations of the T7 promoter (T7) and terminator (T7 term) are indicated. Note that the CAT gene is also translated by an IRES (EMCV IRES).
