FIG. 6.

Extension of the ERAV IRES at the 3′ end does not dramatically effect codon usage. (A) Diagram of AgeI insert in plasmid pE1(245–1299), highlighting the positions of the AUG codons. The initiation codons in frame with the GFP gene are shown (arrows). (B) Immune precipitation with GFP antibody of extracts of vTF7-3-infected BHK-21 cell extracts following transfection with plasmid pE1(245–1299) (1299) (WT), or pE1(245–961)Δ2 (Δ2). Labels to the left refer to the identities of bands in the WT and Δ2 lanes only. The small arrows indicate the locations of the slowest- and fastest-migrating species in lane 1299. Molecular size standards in kilodaltons are shown on the right.