TABLE 1.
Oligonucleotides used to amplify regions of the ERAV 5′-NTR
| Oligonucleotide | Location on genomea | Sequence (5′→3′)b |
|---|---|---|
| TH1-F1 | Sense/1 | TAC CGG TCT TAA GCT TGT ACC TGT AGC |
| TH1-F245 | Sense/245 | CTG TCA CCG GTG AGA GGA GCC CG |
| TH1-F338 | Sense/338 | ATT GAC CGG TCG AAG CGT TGC C |
| TH1-F434 | Sense/434 | CCC GAC CGG TGT ATA GCG CCA GG |
| TH1-F508 | Sense/508 | TGT CAC CGG TCT GAC AAA TGC GGA G |
| TH1-R961 | Antisense/961 | TTT GAC CGG TTG GGG GAC AAA AAC AGT TCT C |
| TH1-R1299 | Antisense/1299 | GAA TAC TGC ATG TAC CGG TTT TGC |
a
Direction of oligonucleotide with respect to the genome/nucleotide number downstream of the poly(C) tract.
b
Nucleotides in boldface indicate an AgeI restriction site.