FIG. 2.

Effects of mutations in the gp41 cytoplasmic tail on glycoprotein incorporation in transfected 293-T cells. Immunoprecipitated proteins from cell lysates (A) and from viral pellets from the same experiment (B) are shown on an SDS–8% polyacrylamide gel. The gp120/p24 and gp41/p24 ratios were calculated after phosphorimaging. (C) Quantitation of glycoprotein incorporation, normalized to WT in each experiment. Data represent averages of 8 to 13 experiments for gp120/p24 and 6 to 9 experiments for gp41/p24. Error bars show standard deviations. Sizes are indicated in kilodaltons.