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. 2024 Jul 12;14:16091. doi: 10.1038/s41598-024-64131-3

Figure 3.

Figure 3

High frequency stimulation partially rescues α-Syn-mediated autophagy impairment. (a) Representative images of SH-SY5Y cells transfected with A53T or pcDNA after stimulation for 2 h and non-stimulated controls, stained with DAPI (blue), α-Syn (red) and P62 (green) (Scale bars = 10 µm) (b) Quantification of P62 fluorescence. n = 3 independent experiments, analyzing 10 cells per condition. Bars represent mean ± s.e.m., ***p < 0.001, ****p < 0.0001, one-way ANOVA, Tukey’s m.c.t. (c) Quantification of P62 average puncta size. n = 3 independent experiments, analyzing 10 cells per condition. Bars represent mean ± s.e.m., ****p < 0.0001, one-way ANOVA, Tukey’s m.c.t. (d) Representative images of SH-SY5Y cells transfected with A53T or pcDNA after stimulation for 2 h and non-stimulated controls, stained with DAPI (blue), α-Syn (red) and LAMP-1 (green (Scale bars = 10 µm) (e) Quantification of LAMP-1 fluorescence. n = 3 independent experiments, analyzing 10 cells per condition. Bars represent mean ± s.e.m., *p < 0.05, ****p < 0.0001, one-way ANOVA, Tukey’s m.c.t. (f) Quantification of LAMP-1 average puncta size. n = 3 independent experiments, analyzing 10 cells per condition. Bars represent mean ± s.e.m., *p < 0.05, one-way ANOVA, Tukey’s m.c.t., ns non-significant.