Fig. 7. Temperature triggers preferential binding of bat antibodies to dead bat epithelial cells.
a Gating strategy and identification of cell death induction in M. myotis epithelial cells (MmNep) by oxidative stress. Dead cells are defined as double positive for annexin V and propidium iodide. b Binding of bat (M. myotis) IgG to alive and dead MmNep cells as a function of temperature. Representative histograms from four independent experiments shown binding of fluorochrome labeled (Alexa FluorTM 647) pooled IgG from M. myotis at 25 μg/ml at 22 °C and 42 °C. c Quantification of bat IgG binding to alive and dead bat epithelial cells. The bars represent average value of MFI ± SD depicting the reactivity of antibodies. The average values were calculated from sample replicates (n = 6 for PBS control and n = 9 for samples incubated with antibodies) of mean fluorescence intensity (MFI) obtained in three independent experiments. Each circle in the graph represents an MFI value. Statistical significance was evaluated by applying Two-way ANOVA Tukey’s multiple comparison test. ****p < 0.0001. Source data are provided as a Source Data file.