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. 2024 Jul 12;15:5853. doi: 10.1038/s41467-024-49793-x

Fig. 1. Small growth effect of plasmid carriage with little influence on chromosomal gene transcription at the population level.

Fig. 1

A Growth curve (OD = 600 nm) showing small differences in growth rates of Pseudomonas putida KT2440 with (blue) and without (orange) pKJK5 (Kruskal‒Wallisχ² = 16.516; p = 4.823e-05) and area under the curve (t = 10.045; p = 1.792e-09; n = 12 independent experiments, see Table S1). Samples were taken at OD0.5 and OD1.5 for bulk transcriptomics or microSPLiT sample preparation (indicated by transparent colored circles following color code of B). B PCA multivariate analysis on transcripts from chromosome-encoded genes at the population level (bulk; n = 4 independent experiments) according to the presence (P +) or absence (P−) of the plasmid and according to ODs (OD0.5 vs. OD1.5). The presence of the plasmid did not significantly discriminate between cell transcriptomes, as confirmed using 10,000 permutations in a two-sided PERmutational Multivariate ANalysis Of VAriance (PERMANOVA; r²plasmid = 0.094; pplasmid = 0.0922; r²OD = 0.253; pOD = 0.001; r²plasmid: OD = 0.035; pplasmid: OD = 0.7). Source data and detailed statistical results are displayed in the Source Data file.