Fig. 1.

Overexpression of FAM84B in PCa is induced by eccDNA. A PCR primer design for the validation of eccDNA circular structures and embedded transcripts. B Outward PCR validation of eccDNA circular structure. C Inward PCR detection of eccDNA-containing transcripts. D–E RT–qPCR and western blot detection of FAM84B expression in PCa cells and HPECs. F The efficiency of shRNAs targeting Lig3 was measured using RT–qPCR. G Effect of shLig3 on eccDNA 3# levels in PCa cells was measured using outward PCR. H Effect of knockdown of Lig3 on FAM84B expression in cells by RT–qPCR. I FAM84B protein expression in PCa and adjacent tissues (n = 38) was examined using IHC. Experiments were repeated three times with multiple wells. The bars indicate SD. *p < 0.05 (paired t-test, one-way/two-way ANOVA)