Fig. 2.

FAM84B overexpression promotes the growth and metastasis of PCa cells. PCa cells were infected with shCtrl, shLig 3 1#, shLig 3 1# + oeCtrl, shLig 3 1# + oeFAM84B. A Detection of FAM84B expression by RT–qPCR. B, C The PCa cell proliferation and DNA synthesis activity were assessed using CCK8 and EdU staining. D, E The migratory and invasion of PCa cells were assessed using transwell assays. F The growth rate of xenograft tumors formed by subcutaneously inoculated PC-3 cells in mice (n = 5). G Lig3, FAM84B, and Ki67 protein expression in xenograft tumors (n = 5) was examined using IHC. H Observation of tumor cell metastasis formed by intracardiac injection of PC-3 cells by bioluminescence imaging (n = 5). Experiments were repeated three times with multiple wells. The bars indicate SD. *p < 0.05 (one-way/two-way ANOVA). Scale bar = 50 μm