Fig. 4.

FAM84B-mediated MYC activates the transcription of WWP1. A MYC protein expression in PCa and adjacent tissues (n = 38) was examined using IHC. B The correlation of MYC expression and FAM84B/WWP1 expression in PCa tissues was analyzed using Pearson’s correlation analysis (n = 38). C The FAM84B, MYC, and WWP1 protein expression in PCa cells in response to overexpression of FAM84B and MYC inhibitor MYCi361 treatment was examined using western blot assays. D The binding ability of MYC in the WWP1 promoter region in PCa cells in response to overexpression of FAM84B and MYC inhibitor MYCi361 treatment was examined using western blot assays. E The transcription of WWP1 promoter in PCa cells in response to overexpression of FAM84B and MYC inhibitor MYCi361 treatment was analyzed using dual-luciferase assays. Experiments were repeated three times with multiple wells. The bars indicate SD. *p < 0.05 (paired t-test, two-way ANOVA)