Fig. 5.

MYC promotes WWP1 expression to drive PCa tumor progression. PCa cells were treated with DMSO, MYCi361, oeCtrl + MYCi361, and oeWWP1 + MYCi361 (A) WWP1 mRNA expression in PCa cells was examined using RT–qPCR. B, C The PCa cell proliferation and DNA synthesis activity were assessed using CCK8 and EdU staining. D The growth rate of xenograft tumors formed by subcutaneously inoculated PC-3 cells in mice treated with MYCi361 or DMSO (n = 5). E MYC, WWP1, and Ki67 protein expression in xenograft tumors (n = 5) was examined using IHC. F, G The migratory and invasive capacity of PCa cells were examined using transwell assays. H Observation of tumor cell metastasis formed by intracardiac injection of PC-3 cells in mice treated with MYCi361 or DMSO by bioluminescence imaging (n = 5). Experiments were repeated three times with multiple wells. The bars indicate SD. *p < 0.05 (one-way/two-way ANOVA). Scale bar = 50 μm