Fig. 6.

Downstream target analysis of WWP1. A Predicted downstream target proteins of E3 ubiquitin ligase WWP1. B PPI network of WWP1 target proteins. C CDKN1B protein expression in PCa tissues and adjacent tissues (n = 38) was examined using IHC. D The correlation of CDKN1B expression and FAM84B/WWP1/MYC expression in PCa tissues was analyzed using Pearson’s correlation analysis (n = 38). E The CDKN1B protein expression in PCa cells in response to overexpression of WWP1 and MYC inhibitor MYCi361 treatment was examined using western blot assays. F The CDKN1B mRNA expression in PCa cells in response to overexpression of WWP1 and MYC inhibitor MYCi361 treatment was examined using RT–qPCR. G Endogenous interaction between CDKN1B and WWP1 in PCa cells and the effect of overexpression of WWP1 on ubiquitination of CDKN1B protein examined using Co-IP. H The effect of overexpression of WWP1 on the stability of CDKN1B protein in PCa cells treated with CHX. Experiments were repeated three times with multiple wells. The bars indicate SD. *p < 0.05 (one-way/two-way ANOVA)