Fig. 7.

CDKN1B is an effector of FAM84B in PCa. PCa cells were treated with oeCtrl, oeFAM84B, oeFAM84B + oeCtrl, and oeFAM84B + oeCDKN1B. A FAM84B and CDKN1B protein expression in PCa cells was examined using western blot assays. B, C The PCa cell proliferation and DNA synthesis activity were assessed using CCK8 and EdU staining. D, E The migratory and invasive capacity of PCa cells were examined using transwell assays. F The growth rate of xenograft tumors formed by subcutaneously inoculated PC-3 cells in mice (n = 5). G Observation of tumor cell metastasis formed by intracardiac injection of PC-3 cells in mice by bioluminescence imaging (n = 5). Experiments were repeated three times with multiple wells. The bars indicate SD. *p < 0.05 (one-way/two-way ANOVA). Scale bar = 50 μm