FIG. 4.

Binding of nectin-1:Fc to gD detected by ELISA. Plates (96 wells) were coated with gD-1(306t) (top) or gD-2(306t) (bottom) or BSA and incubated with the concentrations of nectin-1:Fc indicated. The nectin-1:Fc bound to wells was detected with horseradish peroxidase-conjugated anti-rabbit Fc antibody and substrate. The reaction product was detected spectrophotometrically (optical density at 410 nm [OD₄₁₀]).