FIG. 5.

Binding of gD:Fc to CHO cells expressing nectin-1α (FVB/N form). CHO-K1 cells transfected with nectin-1α-expressing plasmid (pDS101) or control plasmid (pcDNA3) were replated in 96-well plates and exposed to purified HSV-1 gD:Fc that had been preincubated with anti-gD MAbs (or control anti-Myc MAb) or buffer alone. After 30 min, the cells were washed, fixed with formaldehyde-glutaraldehyde, and incubated with biotinylated anti-rabbit Fc antibody followed by streptavidin-conjugated horseradish peroxidase. Peroxidase activity was assayed as a measure of gD:Fc binding to the cell surface. The results shown are means of three determinations with standard deviations indicated. OD₃₇₀, optical density at 370 nm.