FIG. 5.

Electrophoretically separated lysates of cells infected with gD^−/+ virus or rescuants and reacted with a monoclonal antibody to gI (A) or polyclonal antibody to gJ (B). SK-N-SH cells were mock infected or exposed (10 PFU/cell) to wild-type virus, P6 (gD^−/+), or p10 (gD^−/+) or individually to three independently derived gD^+/+ rescuants. The cells were harvested at 18 h after infection, solubilized, subjected to electrophoresis in denaturing gels, and reacted with a monoclonal antibody to U_S7 (gI) or polyclonal antibody to U_S5 (gJ).